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      https://www.ias.ac.in/article/fulltext/jbsc/032/03/0585-0593

    • Keywords

       

      Apoptosis; Autophagy; heat shock; HSF; Hsp; necrosis; tumour cells

    • Abstract

       

      Heat shock response is associated with the synthesis of heat shock proteins (Hsps) which is strictly regulated by different members of heat shock transcription factors (HSFs). We previously reported that a rat histiocytoma, BC-8 failed to synthesize Hsps when subjected to typical heat shock conditions (42°C, 60 min). The lack of Hsp synthesis in these cells was due to a failure in HSF1 DNA binding activity. In the present study we report that BC-8 tumor cells when subjected to heat shock at higher temperature (43°C, 60 min) or incubation for longer time at 42°C, exhibited necrosis characteristics; however, under mild heat shock (42°C, 30 min) conditions cells showed activation of autophagy. Mild heat shock treatment induced proteolysis of HSF1, and under similar conditions we observed an increase in HSF2 expression followed by its enhanced DNA binding activity. Inhibiting HSF1 proteolysis by reversible proteasome inhibition failed to inhibit heat shock induced autophagy. Compromising HSF2 expression but not HSF1 resulted in the inhibition of autophagy, suggesting HSF2 dependent activation of autophagy. We are reporting for the first time that HSF2 is heat inducible and functions in heat shock induced autophagic cell death in BC-8 tumor cells.

    • Author Affiliations

       

      Kolla V, P Rasad1 2 Aftab Taiyab1 D Jyothi1 Usha K Srinivas1 Amere S Sreedhar1

      1. Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
      2. Institute for Animal Pathology Universität Bern, 3001, Bern, Switzerland
    • Dates

       
  • Journal of Biosciences | News

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