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      https://www.ias.ac.in/article/fulltext/jbsc/032/02/0251-0260

    • Keywords

       

      Calcium; calcium-binding protein; EhCaBP; CBL; CIPK; calmodulin; antisense; transgenic

    • Abstract

       

      Entamoeba histolytica contains a novel calcium-binding protein like calmodulin, which was discovered earlier, and we have reported the presence of its homologue(s) and a dependent protein kinase in plants. To understand the functions of these in plants, a cDNA encoding a calcium-binding protein isolated from Entamoeba histolytica (EhCaBP) was cloned into vector pBI121 in antisense orientation and transgenic tobacco plants were raised. These plants showed variation in several phenotypic characters, of which two distinct features, more greenness and leaf thickness, were inherited in subsequent generations. The increase in the level of total chlorophyll in different plants ranged from 60% to 70%. There was no major change in chloroplast structure and in the protein level of D1, D2, LHCP and RuBP carboxylase. These morphological changes were not seen in antisense calmodulin transgenic tobacco plants, nor was the calmodulin level altered in EhCaBP antisense plants.

    • Author Affiliations

       

      Girdhar K Pandey1 2 Amita Pandey3 2 Vanga Siva Reddy1 Renu Deswal4 Alok Bhattacharya3 Kailash C Upadhyaya3 Sudhir K Sopory1

      1. International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India
      2. Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
      3. School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
      4. Department of Botany, University of Delhi, India
    • Dates

       
  • Journal of Biosciences | News

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