• Agrobacterium-mediated transformation of chickpea with α-amylase inhibitor gene for insect resistance

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      https://www.ias.ac.in/article/fulltext/jbsc/031/03/0339-0345

    • Keywords

       

      Agrobacterium ; α-amylase inhibitor; chickpea; insect resistance

    • Abstract

       

      Chickpea is the world’s third most important pulse crop and India produces 75% of the world’s supply. Chickpea seeds are attacked byCallosobruchus maculatus andC. chinensis which cause extensive damage. The α-amylase inhibitor gene isolated fromPhaseolus vulgaris seeds was introduced into chickpea cultivar K850 throughAgrobacterium- mediated transformation. A total of 288 kanamycin resistant plants were regenerated. Only 0.3% of these were true transformants. Polymerase chain reaction (PCR) analysis and Southern hybridization confirmed the presence of 4.9 kb α-amylase inhibitor gene in the transformed plants. Western blot confirmed the presence of α-amylase inhibitor protein. The results of bioassay study revealed a significant reduction in the survival rate of bruchid weevilC. maculatus reared on transgenic chickpea seeds. All the transgenic plants exhibited a segregation ratio of 3:1.

    • Author Affiliations

       

      S Ignacimuthu1 S Prakash1

      1. Entomology Research Institute, Loyola College, Chennai - 600 034, India
    • Dates

       
  • Journal of Biosciences | News

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