• Ni2+-uptake inPseudomonas putida strain S4: a possible role of Mg2+-uptake pump

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      https://www.ias.ac.in/article/fulltext/jbsc/031/01/0061-0067

    • Keywords

       

      CorA; Co(III)Hex; metal uptake; Mg2+; Ni2+

    • Abstract

       

      Essential metal ion homeostasis is based on regulated uptake of metal ions, both during its scarcity and abundance.Pseudomonas putida strain S4, a multimetal resistant bacterium, was employed to investigate Ni2+ entry into cells. It was observed that Mg2+ regulates the entry of Ni2+ and by this plays a protective role to minimize Ni2+ toxicity in this strain. This protection was evident in both growth as well as viability. Intracellular accumulation of Ni2+ varied in accordance with Mg2+ concentrations in the medium. It was hypothesized that Ni2+ enters the cell using a broad Mg2+ pump, i.e. the CorA system, as the CorA inhibitor, i.e. Co(III) Hex, also inhibits Ni2+ uptake. This led to the inference that Mg2+-based protection was basically due to competitive inhibition of Ni2+ uptake. We also show that Zn2+ can further regulate the entry of Ni2+

    • Author Affiliations

       

      V N Tripathi1 S Srivastava1

      1. Department of Genetics, University of Delhi, South Campus, New Delhi - 110 021, India
    • Dates

       
  • Journal of Biosciences | News

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