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    • Keywords


      BoRS1 gene; dry matter accumulation; leaf chlorophyll content; relative water content; tobacco; water stress

    • Abstract


      Water stress is by far the leading environmental stress limiting crop yields worldwide. Genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to water stress. In this study, theBrassica oleracea var.acephala BoRS1 gene was transferred into tobacco throughAgrobacterium- mediated leaf disc transformation. The transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (PCR) analysis, Southern hybridization and semi-quantitative one step RT-PCR analysis respectively. Subsequently, the growth status under water stress, and physiological responses to water stress of transgenic tobacco were studied. The results showed that the transgenic plants exhibited better growth status under water stress condition compared to the untransformed control plants. In physiological assessment of water tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of water stress than the untransformed control plants. This study shows thatBoRS1 is a candidate gene in the engineering of crops for enhanced water stress tolerance.

    • Author Affiliations


      Dongqin Tang1 2 Hongmei Qian2 Lingxia Zhao2 Danfeng Huang1 Kexuan Tang1 2

      1. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai - 201101, People’s Republic of China
      2. Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai - 200030, People’s Republic of China
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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