Frequent outbreaks of the purulence disease of Chinese oak silkworm are reported in Middle and Northeast China. The disease is produced by the pathogenAntheraea pernyi nucleopolyhedrovirus (AnpeNPV). To obtain molecular information of the virus, the polyhedra of AnpeNPV were purified and characterized. The genomic DNA of AnpeNPV was extracted and digested withHindIII. The genome size of AnpeNPV is estimated at 128 kb. Based on the analysis of DNA fragments digested withHindIII, 23 fragments were bigger than 564 bp. A genomic library was generated usingHindIII and the positive clones were sequenced and analysed. Thegp64 gene, encoding the baculovirus envelope protein GP64, was found in an insert. The nucleotide sequence analysis indicated that the AnpeNPVgp64 gene consists of a 1530 nucleotide open reading frame (ORF), encoding a protein of 509 amino acids. Of the eightgp64 homologues, the AnpeNPVgp64 ORF shared the most sequence similarity with thegp64 gene ofAnticarsia gemmatalis NPV, but notBombyx mori NPV. The upstream region of the AnpeNPVgp64 ORF encoded the conserved transcriptional elements for early and late stage of the viral infection cycle. These results indicated that AnpeNPV belongs to group I NPV and was far removed in molecular phylogeny from the BmNPV.
Volume 45, 2020
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