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      https://www.ias.ac.in/article/fulltext/jbsc/030/04/0483-0490

    • Keywords

       

      Alloxan diabetes; antioxidants; Na+/K+ ATPase; sodium orthovanadate (SOV); Trigonella foenum graecum seed powder (TSP)

    • Abstract

       

      Trigonella foenum graecum seed powder (TSP) and sodium orthovanadate (SOV) have been reported to have antidiabetic effects. However, SOV exerts hypoglycemic effects at relatively high doses with several toxic effects. We used low doses of vanadate in combination with TSP and evaluated their antidiabetic effects on antioxidant enzymes and membrane-linked functions in diabetic rat brains. In rats, diabetes was induced by alloxan monohydrate (15 mg/100 g body wt.) and they were treated with 2 IU insulin, 0.6 mg/ml SOV, 5% TSP and a combination of 0.2 mg/ml SOV with 5% TSP for 21 days. Blood glucose levels, activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), Na+/K+ ATPase, membrane lipid peroxidation and fluidity were determined in different fractions of whole brain after 21 days of treatment. Diabetic rats showed high blood glucose (P < 0.001), decreased activities of SOD, catalase and Na+/K+ ATPase (P < 0.01,P < 0.001 andP < 0.01), increased levels of GPx and MDA (P < 0.01 andP < 0.001) and decreased membrane fluidity (P < 0.01). Treatment with different antidiabetic compounds restored the above-altered parameters. Combined dose ofTrigonella and vanadate was found to be the most effective treatment in normalizing these alterations. Lower doses of vanadate could be used in combination with TSP to effectively counter diabetic alterations without any toxic effects.

    • Author Affiliations

       

      Mohammad Rizwan Siddiqui1 Asia Taha1 K Moorthy1 Mohd Ejaz Hussain1 2 S F Basir1 2 Najma Zaheer Baquer1

      1. School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110 067, India
      2. Department Biosciences, Jamia Millia Islamia, New Delhi - 110 025, India
    • Dates

       
  • Journal of Biosciences | News

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