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      https://www.ias.ac.in/article/fulltext/jbsc/030/02/0237-0244

    • Keywords

       

      Annona squamosa seed; cytotoxicity; GSH; ROS; tumour cells

    • Abstract

       

      Annonaceous acetogenins are a new class of compounds that have been reported to have potent pesticidal, parasiticidal, anti-microbial, cell growth inhibitory activities. In this study, organic and aqueous extracts from the defatted seeds ofAnnona squamosa (custard apple) were tested on different human tumour cell lines for antitumoural activity. While organic and aqueous extracts induced apoptosis in MCF-7 and K-562 cells, they failed to do so in COLO-205 cells. Treatment of MCF-7 and K-562 cells with organic and aqueous extracts resulted in nuclear condensation, DNA fragmentation, induction of reactive oxygen species (ROS) generation and reduced intracellular glutathione levels. In addition downregulation of Bcl-2 and PS externalization by Annexin-V staining suggested induction of apoptosis in MCF-7 and K-562 cells by both the extracts through oxidative stress. On the contrary, COLO-205 cells showed only PS externalization but no change in ROS and glutathione levels. These observations suggest that the induction of apoptosis byA. squamosa extracts can be selective for certain types of cancerous cells

    • Author Affiliations

       

      B V V Pardhasaradhi1 Madhurima Reddy1 A Mubarak Ali1 A Leela Kumari1 Ashok Khar1

      1. Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India
    • Dates

       
  • Journal of Biosciences | News

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