• Tissue localization and partial characterization of pheromone biosynthesis activating neuropeptide inAchaea janata

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    • Keywords

       

      Achaea janata ; immunocytochemistry; MALDI-MS; pheromone biosynthesis activating neuropeptide (PBAN); RP-HPLC

    • Abstract

       

      Female sex pheromone production in certain moth species have been shown to be regulated by a cephalic endocrine peptidic factor: pheromone biosynthesis activating neuropeptide (PBAN), having 33 amino acid residues. Antisera against syntheticHeliothis zea-PBAN were developed. Using these polyclonals, immunoreactivity was mapped in the nervous system ofAchaea janata. Three distinct groups of immunopositive secretory neurons were identified in the suboesophageal ganglion; and immunoreactivity was observed in the corpora cardiaca, thoracic and in the abdominal ganglia. From about 6000 brain sub-oesophageal ganglion complexes, the neuropeptide was isolated; and purified sequentially by Sep-pak and reversed phase high performance liquid chromatographic methods. Identity of purified PBAN fraction was confirmed with polyclonal antibody by immunoblotting. Molecular mass of the isolated peptide was determined by matrix-assisted laser desorption/ionization mass spectrometry, and was found to be 3900 Da, same as that of knownH. zea-PBAN. Radiochemical bioassay confirmed the pheromonotropic effect of the isolated neuropeptide in this insect

    • Author Affiliations

       

      V S Ajitha1 D Muraleedharan1

      1. Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram - 695 581, India
    • Dates

       
  • Journal of Biosciences | News

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