• Stochasticity or the fatal ‘imperfection’ of cloning

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/030/01/0021-0030

    • Keywords

       

      Clone; mutation; monoallelic expression; stochasticity

    • Abstract

       

      The concept of clone is analysed with the aim of exploring the limits to which a phenotype can be said to be determined geneticaly. First of all, mutations that result from the replication, topological manipulation or lesion of DNA introduce a source of heritable variation in an otherwise identical genetic background. But more important, stochastic effects in many biological processes may superimpose a phenotypic variation which is not encoded in the genome. The source of stochasticity ranges from the random selection of alleles or whole chromosomes to be expressed in small cell populations, to fluctuations in processes such as gene expression, due to limiting amounts of the players involved. The picture emerging is that the term clone is a statistical over-simplification representing a series of individuals having essentially the same genome but capable of exhibiting wide phenotypic variation. Finally, to what extent fluctuations in biological processes, usually thought of as noise, are in fact signal is also discussed.

    • Author Affiliations

       

      Reiner A Veitia1

      1. Hospital Cochin, Pavillon Baudelocque, Université Denis Diderot/Paris VII INSERM U361 Reproduction et Physiopathologie Obstetricale, 123 Bd de Port Royal, Paris - 75014, France
    • Dates

       
  • Journal of Biosciences | News

© 2017-2019 Indian Academy of Sciences, Bengaluru.