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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/029/04/0399-0407

    • Keywords

       

      Evolution analysis; mannose-binding lectin; molecular modelling; Taxus media ; TMA

    • Abstract

       

      In this paper, we report the cloning and characterization of the first mannose-binding lectin gene from a gymnosperm plant species,Taxus media. The full-length cDNA ofT. media agglutinin (TMA) consisted of 676 bp and contained a 432 bp open reading frame (ORF) encoding a 144 amino acid protein. Comparative analysis showed that TMA had high homology with many previously reported plant mannose-binding lectins and thattma encoded a precursor lectin with a 26-aa signal peptide. Molecular modelling revealed that TMA was a new mannosebinding lectin with three typical mannose-binding boxes like lectins from species of angiosperms. Tissue expression pattern analyses revealed thattma is expressed in a tissue-specific manner in leaves and stems, but not in fruits and roots. Phylogenetic tree analyses showed that TMA belonged to the structurally and evolutionarily closely related monocot mannose-binding lectin superfamily. This study provides useful information to understand the molecular evolution of plant lectins.

    • Author Affiliations

       

      Guoyin Kai1 2 Lingxia Zhao1 2 Jingui Zheng3 Lei Zhang4 5 6 Zhiqi Miao1 2 Xiaofen Sun5 6 Kexuan Tang1 2 5 6

      1. Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai - 200030, People’s Republic of China
      2. Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, School of Life Sciences and Technology, Shanghai Jiao Tong University, Shanghai - 200030, People’s Republic of China
      3. Biotechnology Center, Fujian Agricultural and Forest University, Fuzhou - 350002, People’s Republic of China
      4. School of Pharmacy, Second Military Medical University, Shanghai - 200433, People’s Republic of China
      5. State Key Laboratory of Genetic Engineering, Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, Fudan University, Shanghai - 200433, People’s Republic of China
      6. School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai - 200433, People’s Republic of China

    • Dates

       
      Published
      December 2004
      Manuscript received
      22 June 2004
      Manuscript revised
      29 September 2004

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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