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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/029/04/0399-0407

    • Keywords

       

      Evolution analysis; mannose-binding lectin; molecular modelling; Taxus media ; TMA

    • Abstract

       

      In this paper, we report the cloning and characterization of the first mannose-binding lectin gene from a gymnosperm plant species,Taxus media. The full-length cDNA ofT. media agglutinin (TMA) consisted of 676 bp and contained a 432 bp open reading frame (ORF) encoding a 144 amino acid protein. Comparative analysis showed that TMA had high homology with many previously reported plant mannose-binding lectins and thattma encoded a precursor lectin with a 26-aa signal peptide. Molecular modelling revealed that TMA was a new mannosebinding lectin with three typical mannose-binding boxes like lectins from species of angiosperms. Tissue expression pattern analyses revealed thattma is expressed in a tissue-specific manner in leaves and stems, but not in fruits and roots. Phylogenetic tree analyses showed that TMA belonged to the structurally and evolutionarily closely related monocot mannose-binding lectin superfamily. This study provides useful information to understand the molecular evolution of plant lectins.

    • Author Affiliations

       

      Guoyin Kai1 2 Lingxia Zhao1 2 Jingui Zheng3 Lei Zhang4 5 6 Zhiqi Miao1 2 Xiaofen Sun5 6 Kexuan Tang1 2 5 6

      1. Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai - 200030, People’s Republic of China
      2. Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, School of Life Sciences and Technology, Shanghai Jiao Tong University, Shanghai - 200030, People’s Republic of China
      3. Biotechnology Center, Fujian Agricultural and Forest University, Fuzhou - 350002, People’s Republic of China
      4. School of Pharmacy, Second Military Medical University, Shanghai - 200433, People’s Republic of China
      5. State Key Laboratory of Genetic Engineering, Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, Fudan University, Shanghai - 200433, People’s Republic of China
      6. School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai - 200433, People’s Republic of China
    • Dates

       
  • Journal of Biosciences | News

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