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An allele-specific polymerase chain reaction assay for the differentiation of members of theAnopheles culicifacies complex
O P Singh Geeta Goswami N Nanda K Raghavendra D Chandra S K Subbarao
Volume 29 Issue 3 September 2004 pp 275-280
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https://www.ias.ac.in/article/fulltext/jbsc/029/03/0275-0280 -
Keywords
Anopheles culicifacies ;cryptic species ;malaria ;polymerase chain reaction ;ribosomal DNA ;species complex ;vector -
Abstract
Anopheles culicifacies, the principal vector of malaria in India, is a complex of five cryptic species which are morphologically indistinguishable at any stage of life. In view of the practical difficulties associated with classical cytotaxonomic method for the identification of members of the complex, an allele-specific polymerase chain reaction (ASPCR) assay targeted to the D3 domain of 28S ribosomal DNA was developed. The assay discriminatesAn. culicifacies species A and D from species B, C and E. The assay was validated using chromosomally-identified specimens ofAn. culicifacies from different geographical regions of India representing different sympatric associations. The assay correctly differentiates species A and D from species B, C and E. The possible use of this diagnostic assay in disease vector control programmes is discussed.
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Author Affiliations
O P Singh1 Geeta Goswami1 N Nanda1 K Raghavendra1 D Chandra1 S K Subbarao1 2
- Malaria Research Centre (ICMR), 22 Sham Nath Marg, Delhi - 110 054, India
- Indian Council of Medical Research, Ramalingaswami Bhawan, Ansari Nagar, New Delhi - 110 029, India
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Dates
- Published
- September 2004
- Manuscript received
- 8 December 2003
- Accepted
- 8 May 2004
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Journal of Biosciences
Volume 48, 2023
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Journal of Biosciences | News
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To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".
Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.
Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.
Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx
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