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    • Keywords


      Genome data analysis; hypothetical proteins,Mycobacterium tuberculosis; protein structures; structural and functional domains

    • Abstract


      The sequencing of theMycobacterium tuberculosis (MTB) H37Rv genome has facilitated deeper insights into the biology of MTB, yet the functions of many MTB proteins are unknown. We have used sensitive profile-based search procedures to assign functional and structural domains to infer functions of gene products encoded in MTB. These domain assignments have been made using a compendium of sequence and structural domain families. Functions are predicted for 78% of the encoded gene products. For 69% of these, functions can be inferred by domain assignments. The functions for the rest are deduced from their homology to proteins of known function. Superfamily relationships between families of unknown and known structures have increased structural information by ∼ 11%. Remote similarity detection methods have enabled domain assignments for 1325 ‘hypothetical proteins’. The most populated families in MTB are involved in lipid metabolism, entry and survival of the bacillus in host. Interestingly, for 353 proteins, which we refer to as MTB-specific, no homologues have been identified. Numerous, previously unannotated, hypothetical proteins have been assigned domains and some of these could perhaps be the possible chemotherapeutic targets. MTB-specific proteins might include factors responsible for virulence. Importantly, these assignments could be valuable for experimental endeavors. The detailed results are publicly available at http://hodgkin.mbu.iisc.ernet.in/∼dots.

    • Author Affiliations


      Seema Namboori1 Natasha Mhatre1 2 Sentivel Sujatha1 3 Narayanaswamy Srinivasan1 Shashi Bhushan Pandit1

      1. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012, India
      2. Center for Ecological Sciences, Indian Institute of Science, Bangalore - 560 012, India
      3. Reddy US Therapeutics, Norcross, GA - 30071, USA
    • Dates

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