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      https://www.ias.ac.in/article/fulltext/jbsc/029/01/0067-0071

    • Keywords

       

      Cotton (Gossypium); gossypol; high-performance liquid chromatography (HPLC); pigment gland

    • Abstract

       

      A comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through an optimized high-performance liquid chromatography (HPLC) on a C18 column (4.6 mm x 250 mm, 5 Μm particle) with methanol-0.5% acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and UV detection at 254 nm. The method was shown to be highly reproducible, with precision [as relative standard deviation (RSD)] and accuracy [as relative mean error (RME)] < 10%, both intra-day and inter-day. Absolute recoveries were > 94%. The results revealed major differences among the different gland varieties or species of cotton, including the special and ordinary glandless and glandedGossypium hirsutum, G. barbadense, and displayed the precious resources of different glands of extraordinary cotton

    • Author Affiliations

       

      Yingfan Cai1 2 Hong Zhang2 Yu Zeng2 Jianchuan Mo1 Jinku Bao2 Chen Miao2 Jie Bai2 Fang Yan2 Fang Chen2

      1. College of Bioinformation, Chongqing University of Post and Telecom, Chongqing - 400065, People’s Republic of China
      2. College of Life Science, Sichuan University, Chengdu - 610064, People’s Republic of China
    • Dates

       
  • Journal of Biosciences | News

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