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Middle silkgland tissue; proteome; two-dimensional polyacrylamide gel electrophoresis; yellow cocoon sex-limited variety

Qualitative and quantitative differences in proteins expressed in the middle silkglands of male and female silkworm larvae that differ in silk colour were investigated by high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), followed by computer assisted image analysis. About 1000 protein spots were resolved in both the sexes and most proteins were shown to be distributed in the area from 15 kDa to 70 kDa and pH 4–8. It was found that some proteins displayed higher expression in yellow cocoon, while two proteins were only expressed in female silkworm silkgland tissue through the comparison and analysis by two-D software. These proteins especially existed in female silkworm middle silkgland tissue of yellow cocoon. Furthermore, these proteins might be involved in the expression of cocoon colour phenotype

Yuan-Xiang Jin¹ Yu-Yin Chen¹ Meng-Kui Xu¹ Yong-Huang Jiang¹

1. College of Animal Sciences, Zhejiang University, Hangzhou - 310029, People’s Republic of China

Published

March 2004

Manuscript received

10 August 2003

Accepted

6 January 2004