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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/029/01/0045-0049

    • Keywords

       

      Middle silkgland tissue; proteome; two-dimensional polyacrylamide gel electrophoresis; yellow cocoon sex-limited variety

    • Abstract

       

      Qualitative and quantitative differences in proteins expressed in the middle silkglands of male and female silkworm larvae that differ in silk colour were investigated by high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), followed by computer assisted image analysis. About 1000 protein spots were resolved in both the sexes and most proteins were shown to be distributed in the area from 15 kDa to 70 kDa and pH 4–8. It was found that some proteins displayed higher expression in yellow cocoon, while two proteins were only expressed in female silkworm silkgland tissue through the comparison and analysis by two-D software. These proteins especially existed in female silkworm middle silkgland tissue of yellow cocoon. Furthermore, these proteins might be involved in the expression of cocoon colour phenotype

    • Author Affiliations

       

      Yuan-Xiang Jin1 Yu-Yin Chen1 Meng-Kui Xu1 Yong-Huang Jiang1

      1. College of Animal Sciences, Zhejiang University, Hangzhou - 310029, People’s Republic of China
    • Dates

       
  • Journal of Biosciences | News

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