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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/029/01/0015-0022

    • Keywords

       

      Antisense; cytokinesis; gp63; Leishmania

    • Abstract

       

      The major surface glycoprotein gp63 of the kinetoplastid protozoal parasiteLeishmania is implicated as a ligand mediating uptake of the parasite into, and survival within, the host macrophage. By expressing gp63 antisense RNA from an episomal vector inL. donovani promastigotes, gp63-deficient transfectants were obtained. Reduction of the gp63 level resulted in increased generation times, altered cell morphology, accumulation of cells in the G2/M phase of the cell cycle, and increased numbers of binucleate cells with one or two kinetoplasts. Growth was stimulated, and the number of binucleate cells reduced, by addition to the culture of a bacterially expressed fusion protein containing the fibronectin-like SRYD motif and the zinc-binding (metalloprotease) domain of gp63. These observations support an additional role of gp63 in promastigote multiplication; the fibronectin-like properties of gp63 may be important in this process

    • Author Affiliations

       

      Sanjeev Pandey1 2 Phuljhuri Chakraborti1 Rakhi Sharma1 Santu Bandyopadhyay1 Dwijen Sarkar1 Samit Adhya1

      1. Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata - 700 032, India
      2. BB College, Asansol, Dist., Burdwan, Asansol - 713 303, India
    • Dates

       
  • Journal of Biosciences | News

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