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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/029/01/0015-0022

    • Keywords

       

      Antisense; cytokinesis; gp63; Leishmania

    • Abstract

       

      The major surface glycoprotein gp63 of the kinetoplastid protozoal parasiteLeishmania is implicated as a ligand mediating uptake of the parasite into, and survival within, the host macrophage. By expressing gp63 antisense RNA from an episomal vector inL. donovani promastigotes, gp63-deficient transfectants were obtained. Reduction of the gp63 level resulted in increased generation times, altered cell morphology, accumulation of cells in the G2/M phase of the cell cycle, and increased numbers of binucleate cells with one or two kinetoplasts. Growth was stimulated, and the number of binucleate cells reduced, by addition to the culture of a bacterially expressed fusion protein containing the fibronectin-like SRYD motif and the zinc-binding (metalloprotease) domain of gp63. These observations support an additional role of gp63 in promastigote multiplication; the fibronectin-like properties of gp63 may be important in this process

    • Author Affiliations

       

      Sanjeev Pandey1 2 Phuljhuri Chakraborti1 Rakhi Sharma1 Santu Bandyopadhyay1 Dwijen Sarkar1 Samit Adhya1

      1. Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata - 700 032, India
      2. BB College, Asansol, Dist., Burdwan, Asansol - 713 303, India

    • Dates

       
      Published
      March 2004

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

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