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    • Calcium regulates the expression of aDictyostelium discoideum asparaginyl tRNA synthetase gene

      Jyoti K Jaiswal Vidyanand Nanjundiah

      Articles Volume 28 Issue 6 December 2003 pp 697-707

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/028/06/0697-0707

    • Keywords

       

      Calcium; cell cycle; Dictyostelium ; RAP-PCR; tRNA synthetase

    • Abstract

       

      In a screen for calcium-regulated gene expression during growth and development ofDictyostelium discoideum we have identified an asparaginyl tRNA synthetase (ddAsnRS) gene, the second tRNA synthetase gene identified in this organism. TheddAsnRS gene shows many unique features. One, it is repressed by lowering cellular calcium, making it the first known calcium-regulated tRNA synthetase. Two, despite the calcium-dependence, its expression is unaltered during the cell cycle, making this the firstD. discoideum gene to show a calcium-dependent but cell cycle phase-independent expression. Finally, the N-terminal domain of the predicted ddAsnRS protein shows higher sequence similarity to Glutaminyl tRNA synthetases than to other Asn tRNA synthetases. These unique features of theAsnRS from this primitive eukaryote not only point to a novel mechanism regulating the components of translation machinery and gene expression by calcium, but also hint at a link between the evolution ofGlnRS andAsnRS in eukaryotes.

    • Author Affiliations

       

      Jyoti K Jaiswal1 2 Vidyanand Nanjundiah1

      1. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore - 560 012, India
      2. The Rockefeller University, 1230 York Avenue, Box 304, New York, NY - 10021, USA

    • Dates

       
      Published
      December 2003
      Manuscript received
      22 July 2003
      Accepted
      22 October 2003

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