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    • The expression of GFP under the control of fibroin promotor in primary ovarian cells ofAntheraea pernyi

      Wenli Li Liji Jin Pengcheng Bu Lijia An

      Articles Volume 28 Issue 6 December 2003 pp 691-695

    • Fulltext

       

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      https://www.ias.ac.in/article/fulltext/jbsc/028/06/0691-0695

    • Keywords

       

      Antheraea pernyi ; expression; fibroin gene; GFP; transcription initiation site

    • Abstract

       

      The fibroin promoter can stably express foreign gene in lepidopteran cells. Total RNA was extracted from the gland of silkworm,Antheraea pernyi and the transcription initiation site of fibroin gene ofA. pernyi was identified by RNA ligase mediated rapid amplification of cDNA ends (RLM-RACE). The expression vector (pGFP-N2/Fib) was constructed by use of replacing the CMV promoter with the fibroin promoter. The results of visual screening under a fluorescent inverted microscope and Western blot analysis indicated that the GFP gene was expressed in the primary cells of ovary origins fromA. pernyi.

    • Author Affiliations

       

      Wenli Li1 Liji Jin1 Pengcheng Bu1 Lijia An1

      1. Bioengineering Department, Dalian University of Technology, Dalian - 116012, People’s Republic of China

    • Dates

       
      Published
      December 2003
      Manuscript received
      19 March 2003
      Accepted
      16 August 2003

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      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


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