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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/028/04/0437-0442

    • Keywords

       

      Actinorhizal association; Alnus ; Frankia ; molecular marker; rrn ITS

    • Abstract

       

      While the ribosomal RNA like highly conserved genes are good molecular chronometers for establishing phylogenetic relationships, they can also be useful in securing the amplification of adjoining hyper-variable regions. These regions can then be used for developing specific PCR primers or PCR-RFL profiles to be used as molecular markers. We report here the use of ITS region ofrrn operon ofFrankia for developing PCR-RFL profiles capable of discriminating between closely related frankiae. We have also made use of the ITS 1 region of the nuclearrrn operon ofAlnus nepalensis (D Don) for designing a PCR primer for specific amplification of nuclear DNA of this tree.

    • Author Affiliations

       

      Rajani Varghese1 Vineeta S Chauhan1 Arvind K Misra1

      1. Department of Botany, North-Eastern hill University, Shillong - 793 022, India
    • Dates

       
  • Journal of Biosciences | News

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