• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/028/04/0423-0436

    • Keywords

       

      Activation; gene regulation; GAL4-VP16; transcription; yTBP dimers

    • Abstract

       

      Binding characteristics of yeast TATA-binding protein (yTBP) over five oligomers having different TATA variants and lacking a UASGal, showed that TATA-binding protein (TBP)-TATA complex gets stabilized in the presence of the acidic activator GAL4-VP16. Activator also greatly suppressed the non-specific TBP-DNA complex formation. The effects were more pronounced over weaker TATA boxes. Activator also reduced the TBP dimer levels bothin vitro andin vivo, suggesting the dimer may be a direct target of transcriptional activators. The transcriptional activator facilitated the dimer to monomer transition and activated monomers further to help TBP bind even the weaker TATA boxes stably. The overall stimulatory effect of the GAL4-VP16 on the TBP-TATA complex formation resembles the known effects of removal of the N-terminus of TBP on its activity, suggesting that the activator directly targets the N-terminus of TBP and facilitates its binding to the TATA box.

    • Author Affiliations

       

      Anurag Kumar Mishra1 Perumal Vanathi1 Purnima Bhargava1

      1. Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India
    • Dates

       
  • Journal of Biosciences | News

© 2017 Indian Academy of Sciences, Bengaluru.