• Bioluminometric assay of ATP in mouse brain: Determinant factors for enhanced test sensitivity

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/028/04/0379-0382

    • Keywords

       

      ATP assay; bioluminescence; mouse brain

    • Abstract

       

      Firefly luciferase bioluminescence (FLB) is a highly sensitive and specific method for the analysis of adenosine-5-triphosphate (ATP) in biological samples. Earlier attempts to modify the FLB test for enhanced sensitivity have been typically based onin vitro cell systems. This study reports an optimized FLB procedure for the analysis of ATP in small tissue samples. The results showed that the sensitivity of the FLB test can be enhanced several fold by using ultraturax homogenizer, perchloric acid extraction, neutralization of acid extract and its optimal dilution, before performing the assay reaction.

    • Author Affiliations

       

      Haseeb Ahmad Khan1

      1. Department of Medical Research, Riyadh Al Kharj Hospital Program, Riyadh - 11159, Kingdom of Saudi Arabia
    • Dates

       
  • Journal of Biosciences | News

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