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    • Bioluminometric assay of ATP in mouse brain: Determinant factors for enhanced test sensitivity

      Haseeb Ahmad Khan

      Brief Communication Volume 28 Issue 4 June 2003 pp 379-382

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/028/04/0379-0382

    • Keywords

       

      ATP assay; bioluminescence; mouse brain

    • Abstract

       

      Firefly luciferase bioluminescence (FLB) is a highly sensitive and specific method for the analysis of adenosine-5-triphosphate (ATP) in biological samples. Earlier attempts to modify the FLB test for enhanced sensitivity have been typically based onin vitro cell systems. This study reports an optimized FLB procedure for the analysis of ATP in small tissue samples. The results showed that the sensitivity of the FLB test can be enhanced several fold by using ultraturax homogenizer, perchloric acid extraction, neutralization of acid extract and its optimal dilution, before performing the assay reaction.

    • Author Affiliations

       

      Haseeb Ahmad Khan1

      1. Department of Medical Research, Riyadh Al Kharj Hospital Program, Riyadh - 11159, Kingdom of Saudi Arabia

    • Dates

       
      Published
      June 2003
      Manuscript received
      31 January 2003
      Accepted
      13 March 2003

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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