• Purification and characterization of a Ca2+-dependent/calmodulin-stimulated protein kinase from moss chloronema cells

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      https://www.ias.ac.in/article/fulltext/jbsc/028/02/0223-0233

    • Keywords

       

      Calcium-dependent/calmodulin-stimulated protein kinase; chloronema; Funaria hygrometrica ; moss CCaMK

    • Abstract

       

      We have demonstrated the presence of a Ca2+-dependent/calmodulin-stimulated protein kinase (PK) in chloronema cells of the mossFunaria hygrometrica. The kinase, with a molecular mass of 70,000 daltons (PK70), was purified to homogeneity using ammonium sulphate fractionation, DEAE-cellulose chromatography, and calmodulin (CaM)-agarose affinity chromatography. The kinase activity was stimulated at a concentration of 50 (AM free Ca2+, and was further enhanced 3–5-fold with exogenously added 3–1000 nm moss calmodulin (CaM). Autophosphorylation was also stimulated with Ca2+ and CaM. Underin vitro conditions, PK70 phosphorylated preferentially lysine-rich substrates such as HIIIS and HVS. This PK shares epitopes with the maize Ca2+-dependent/calmodulin-stimulated PK (CCaMK) and also exhibits biochemical properties similar to the maize, lily, and tobacco CCaMK. We have characterized it as a moss CCaMK.

    • Author Affiliations

       

      Jacinta S D’Souza1 Man Mohan Johri1

      1. Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai - 400 005, India
    • Dates

       
  • Journal of Biosciences | News

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