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    • Evidence for lipopolysaccharideinduced differentiation of RAW264.7 murine macrophage cell line into dendritic like cells

      Rajiv K Saxena Val Vallyathan Daniel M Lewis

      Volume 28 Issue 1 February 2003 pp 129-134

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/028/01/0129-0134

    • Keywords

       

      Dendritic cells; differentiation; flow cytometry; lipopolysaccharide

    • Abstract

       

      Effect of lipopolysaccharide (LPS) on RAW264.7 macrophage cell line was studied. LPS-treated RAW264.7 cells increased in cell size and acquired distinct dendritic morphology. At the optimal dose of LPS (1 μg/ml), almost 70% RAW264.7 cells acquired dendritic morphology. Flow cytometric studies indicate that the cell surface markers known to be expressed on dendritic cells and involved in antigen presentation and T cell activation (B7.1, B7.2, CD40, MHC class II antigens and CD1d) were also markedly upregulated on LPS-treated RAW264.7 cells. Our results suggest the possibility that LPS by itself could constitute a sufficient signal for differentiation of macrophages into DC-like cells.

    • Author Affiliations

       

      Rajiv K Saxena1 2 Val Vallyathan2 Daniel M Lewis2

      1. School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110 067, India
      2. Analytical Services Branch, HELD, National Institute for Occupational Safety and Health, Center of Disease Control and Prevention, Morgantown, WV - 26506, USA

    • Dates

       
      Published
      February 2003

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