• Characterization ofEntamoeba histolytica-induced dephosphorylation in Jurkat cells

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      https://www.ias.ac.in/article/fulltext/jbsc/027/06/0615-0618

    • Keywords

       

      Apoptosis; CD45; Entamoeba moshkovskii ; Jurkat cells

    • Abstract

       

      Entamoeba histolytica killing of host cells is contact dependent and mediated by a Gal/GalNAc lectin. Upon contact with amoeba a rapid and extensive dephosphorylation of tyrosine phosphorylated host cell proteins is observed. This effect is mediated by the Gal/GalNAc lectin. However, it requires intact cells, as purified lectin failed to induce dephosphorylation in Jurkat cells. The nonpathogenic, but morphologically identical amoeba,Entamoeba moshkovskii also did not induce dephosphorylation in target cells. Treatment of Jurkat cells with phosphotyrosine phosphatase inhibitors has shown that a host phosphatase is responsible for dephosphorylation. However, it was found that the CD45 phosphotase was not necessary for dephosphorylation of host cell proteins.

    • Author Affiliations

       

      J E Teixeira1 B J Mann1 2

      1. Department of Internal Medicine, University of Virginia, Charlottesville, VA - 22908, USA
      2. Department of Microbiology, University of Virginia, Charlottesville, VA - 22908, USA
    • Dates

       
  • Journal of Biosciences | News

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