• Investigating amoebic pathogenesis usingEntamoeba histolytica DNA microarrays

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      https://www.ias.ac.in/article/fulltext/jbsc/027/06/0595-0601

    • Keywords

       

      DNA microarrays; Entamoeba dispar ; Entamoeba histolyticas ; genomic; pathogenesis

    • Abstract

       

      Entamoeba histolytica, a protozoan parasite, causes diarrhea and liver abscesses resulting in 50 million cases of infection worldwide annually. Elucidation of parasite virulence determinants has recently been investigated using genetic approaches. We have undertaken a genomics approach to identify novel virulence determinants in the parasite. A DNA microarray ofE. histolytica is being developed based on sequenced genomic clones from the genome sequencing efforts of The Institute of Genomic Research (TIGR) and the Sanger Center. Hybridization of the slides with samples labelled differentially using fluorescent dyes allows the characterization of transcriptional profiles of genes under the biological conditions tested. Additionally, a genome-wide comparison ofE. histolytica andE. dispar can be undertaken. The development of anE. histolytica microarray will be outlined and its uses in identifying novel virulence determinants and characterizing amoebic biology will be discussed.

    • Author Affiliations

       

      Upinder Singh1 2 Preetam Shah1

      1. Department of Internal Medicine, Stanford University, Stanford - CA 94305, USA
      2. Department of Microbiology, Stanford University, Stanford - CA 94305, USA
    • Dates

       
  • Journal of Biosciences | News

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