• Comparative analysis of naturally occurring L-amino acid osmolytes and their D-isomers on protection ofEscherichia coli against environmental stresses

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      https://www.ias.ac.in/article/fulltext/jbsc/027/05/0515-0520

    • Keywords

       

      Escherichia coli ; D-amino acids; L-amino acids; osmolyte; stress

    • Abstract

       

      Adaptation to high salinity and low or high temperature is essential for bacteria to survive. Accumulation of exogenous osmolytes is one of the ways that helps bacteria to survive under such extracellular stress. We have analysed the capability of various L-amino acids and their D-isomers to act as osmolytes and thus enableEscherichia coli cells to survive under various stress conditions.E. coli cells were grown in the presence or absence of L-and D-proline, alanine, serine and lysine under salt, heat and cold stresses. Of the various amino acids tested, L-proline, closely followed by L-serine turned out to be highly protective against environmental stresses. L-proline provided excellent protection (95%) against salt stress, followed by cold (60%) and heat (40%) stresses. D-amino acids on the other hand, proved to be highly inhibitory under stress conditions. Thus L-amino acids were found to be growth protectants under stress while their D-isomers were inhibitory during stress as well as normal conditions.

    • Author Affiliations

       

      Hanief Md Shahjee1 Kakoli Banerjee2 Faizan Ahmad1

      1. Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi - 110 025, India
      2. Eukaryotic Gene Expression Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi - 110 067, India
    • Dates

       
  • Journal of Biosciences | News

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