• Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/027/03/0261-0272

    • Keywords

       

      cDNA cloning; growth hormone; Indian major carps; overexpression; protein 3-D structure; RACE

    • Abstract

       

      A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences ofLabeo rohita, Cirrhina mrigala andCatla catla have been cloned, characterized and overexpressed inEscherichia coli. These sequences show 96–98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.

    • Author Affiliations

       

      T Venugopal1 S Mathavan1 T J Pandian1

      1. Center for Advanced Studies in Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai - 625 021, India
    • Dates

       
  • Journal of Biosciences | News

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