1. Home
    2. Journals
    3. Journal of Biosciences
    4. Volume 27
    5. Issue 3

    • Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

      T Venugopal S Mathavan T J Pandian

      Volume 27 Issue 3 June 2002 pp 261-272

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/027/03/0261-0272

    • Keywords

       

      cDNA cloning; growth hormone; Indian major carps; overexpression; protein 3-D structure; RACE

    • Abstract

       

      A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences ofLabeo rohita, Cirrhina mrigala andCatla catla have been cloned, characterized and overexpressed inEscherichia coli. These sequences show 96–98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.

    • Author Affiliations

       

      T Venugopal1 S Mathavan1 T J Pandian1

      1. Center for Advanced Studies in Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai - 625 021, India

    • Dates

       
      Published
      June 2002
      Manuscript received
      24 July 2001
      Accepted
      28 March 2002

  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2022-2023 Indian Academy of Sciences, Bengaluru.

Contact | Site index