• Genes encoding chimeras ofNeurospora crassa erg-3 and human TM7SF2 proteins fail to complement Neurospora and yeast sterol C-14 reductase mutants

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/027/02/0105-0112

    • Keywords

       

      Lamin B receptor; sterol reductase

    • Abstract

       

      The human gene TM7SF2 encodes a polypeptide (SR-1) with high sequence similarity to sterol C-14 reductase, a key sterol biosynthetic enzyme in fungi, plants and mammals. In Neurospora and yeast this enzyme is encoded by theerg-3 anderg24 genes respectively. In an effort to demonstrate sterol C-14 reductase activity for SR-1 we constructed six recombinant genes coding for chimeras of the Neurosporaerg-3 and SR-1 protein sequences and tested them for complementation of the Neurosporaerg-3 mutant. To our surprise, all the chimeras failed to complementerg-3. A few of the chimeric proteins were also tested against the yeasterg24 mutant, but again there was no complementation. We discuss some reasons that might account for these unexpected findings

    • Author Affiliations

       

      A Prakash1 Durgadas P Kasbekar1

      1. Centre for Cellular and Molecular Biology, Hyderabad - 500 007, India
    • Dates

       
  • Journal of Biosciences | News

© 2017-2019 Indian Academy of Sciences, Bengaluru.