1. Home
    2. Journals
    3. Journal of Biosciences
    4. Volume 27
    5. Issue 2

    • Genes encoding chimeras ofNeurospora crassa erg-3 and human TM7SF2 proteins fail to complement Neurospora and yeast sterol C-14 reductase mutants

      A Prakash Durgadas P Kasbekar

      Volume 27 Issue 2 March 2002 pp 105-112

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/027/02/0105-0112

    • Keywords

       

      Lamin B receptor; sterol reductase

    • Abstract

       

      The human gene TM7SF2 encodes a polypeptide (SR-1) with high sequence similarity to sterol C-14 reductase, a key sterol biosynthetic enzyme in fungi, plants and mammals. In Neurospora and yeast this enzyme is encoded by theerg-3 anderg24 genes respectively. In an effort to demonstrate sterol C-14 reductase activity for SR-1 we constructed six recombinant genes coding for chimeras of the Neurosporaerg-3 and SR-1 protein sequences and tested them for complementation of the Neurosporaerg-3 mutant. To our surprise, all the chimeras failed to complementerg-3. A few of the chimeric proteins were also tested against the yeasterg24 mutant, but again there was no complementation. We discuss some reasons that might account for these unexpected findings

    • Author Affiliations

       

      A Prakash1 Durgadas P Kasbekar1

      1. Centre for Cellular and Molecular Biology, Hyderabad - 500 007, India

    • Dates

       
      Published
      March 2002
      Manuscript received
      8 November 2001
      Accepted
      9 January 2002

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
      <

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2022-2023 Indian Academy of Sciences, Bengaluru.

Contact | Site index