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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/026/03/0341-0347

    • Keywords

       

      GVBD; insulin; meiotic arrest

    • Abstract

       

      Meiotic arrest of oocyte in an Indian carp,Labeo rohita Ham. has been found for the first time to be withdrawn by insulin only. Addition of insulin to oocytesin vitro caused germinal vesicle breakdown (GVBD), one of the first visual markers to determine initiation of the final maturational process. Under the influence of insulin the germinal vesicle (GV) of the oocyte migrated towards the animal pole, reached the micropyle and then dissolved (GVBD). By using different concentrations of insulin i.e., 0.063, 0.63, 6.3 and 12.6 (AM, optimum amount required was found to be 6.3 (AM. Induction of GVBD by insulin could be blocked by cycloheximide (Chx), a translation inhibitor, while actinomycin D (AcD) had no effect suggesting non-involvement of transcriptional activity in this process. Addition of the maturation-inducing steroid 17α,20β-dihydroxy-4-pregnen-3-one (DHP) stimulated (P < 0.01) GVBD of carp oocytes and its combination with insulin showed an additive effect. Gonadotropin (GtH) caused GVBD but its effect was greatly augmented by insulin. Our results demonstrate that not only can insulin alone induce GVBD in carp oocytes, but it also augments the stimulatory effect of DHP or IGF-I or GtH on GVBD. This information will be important in hormonal manipulation during induced breeding of carp.

    • Author Affiliations

       

      S Dasgupta1 D Basu1 2 L Ravi Kumar1 2 S Bhattacharya1 3

      1. Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar - 751 002, India
      2. Department of Zoology, School of Life Sciences, Visva-Bharati University, Santiniketan - 731 235, India
      3. Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Jadavpur, Kolkata - 700 032, India

    • Dates

       
      Published
      September 2001
      Manuscript received
      28 May 2001
      Accepted
      30 July 2001

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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