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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/026/03/0315-0324

    • Keywords

       

      cDNA cloning; growth hormone; expression inE. coli; Heteropneustes fossilis ; Indian catfish; RT-PCR; sequencing; zebrafish

    • Abstract

       

      A tissue-specific cDNA library was constructed using polyA+ RNA from pituitary glands of the Indian catfishHeteropneustes fossilis (Bloch) and a cDNA clone encoding growth hormone (GH) was isolated. Using polymerase chain reaction (PCR) primers representing the conserved regions of fish GH sequences the 3′ region of catfish GH cDNA (540 bp) was cloned by random amplification of cDNA ends and the clone was used as a probe to isolate recombinant phages carrying the full-length cDNA sequence. The full-length cDNA clone is 1132 bp in length, coding for an open reading frame (ORF) of 603 bp; the reading frame encodes a putative polypeptide of 200 amino acids including the signal sequence of 22 amino acids. The 5′ and 3′ untranslated regions of the cDNA are 58 bp and 456 bp long, respectively. The predicted amino acid sequence ofH. fossils GH shared 98% homology with other catfishes. Mature GH protein was efficiently expressed in bacterial and zebrafish systems using appropriate expression vectors. The successful expression of the cloned GH cDNA of catfish confirms the functional viability of the clone.

    • Author Affiliations

       

      Vikas Anathy1 Thayanithy Venugopal1 Ramanathan Koteeswaran1 Thavamani J Pandian1 Sinnakaruppan Mathavan1 2

      1. Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, Madurai - 625 021, India
      2. Genome Institute of Singapore, National University of Singapore, 1, Research Link, IMA Building No. 04-01, Singapore - 117 604

    • Dates

       
      Published
      September 2001
      Manuscript received
      30 April 2001
      Accepted
      23 July 2001
      Final version published online
      18 September 2007

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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