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    • Sequence analysis of mitochondrial 16S ribosomal RNA gene fragment from seven mosquito species

      Yogesh S Shouche Milind S Patole

      Volume 25 Issue 4 December 2000 pp 361-366

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/025/04/0361-0366

    • Keywords

       

      Mitochondrial 16S rRNA; mosquito; polymerase chain reaction; sequence analysis

    • Abstract

       

      Mosquitoes are vectors for the transmission of many human pathogens that include viruses, nematodes and protozoa. For the understanding of their vectorial capacity, identification of disease carrying and refractory strains is essential. Recently, molecular taxonomic techniques have been utilized for this purpose. Sequence analysis of the mitochondrial 16S rRNA gene has been used for molecular taxonomy in many insects. In this paper, we have analysed a 450 bp hypervariable region of the mitochondrial 16S rRNA gene in three major genera of mosquitoes,Aedes, Anopheles andCulex. The sequence was found to be unusually A + T rich and in substitutions the rate of transversions was higher than the transition rate. A phylogenetic tree was constructed with these sequences. An interesting feature of the sequences was a stretch of Ts that distinguished betweenAedes andCulex on the one hand, andAnopheles on the other. This is the first report of mitochondrial rRNA sequences from these medically important genera of mosquitoes.

    • Author Affiliations

       

      Yogesh S Shouche1 Milind S Patole1

      1. National Center for Cell Science, Pune University Campus, Pune - 411 007, India

    • Dates

       
      Published
      December 2000
      Manuscript received
      27 June 2000
      Accepted
      20 September 2000

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    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

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