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BRCA1 ; breast cancer; microsatellite polymorphisms; tumour suppressive function

The involvement of the familial breast-ovarian cancer gene (BRCA1) in the molecular pathogenesis of breast cancer among Indian women is unknown. We have used a set of microsatellite polymorphisms to examine the frequency of allele loss at the BRCA1 region on chromosome 17q21, in a panel of 80 human breast tumours. Tumour and blood leukocyte/normal tissue DNA from a series of 80 patients with primary breast cancer was screened by PCR-amplified microsatellite length polymorphisms to detect deletions at three polymorphic BRCA1 loci. PCR-allelotype was valuable in examining allele losses from archival and small tumour samples. Loss of alleles at BRCA1 in the patient set, confirmed a noteworthy role of this gene in the molecular pathogenesis of breast cancer and was in accordance with its well-documented tumour suppressive function.

P H Pestonjamasp¹ I Mittra^{1 2}

1. Division of Laboratory Medicine, Tata Memorial Hospital, Ernest Borges Road, Parel, Mumbai - 400 012, India
2. Department of Surgery, Tata Memorial Hospital, Ernest Borges Road, Parel, Mumbai - 400 012, India

Published

March 2000

Manuscript received

14 June 1999

Accepted

19 November 1999