• High level expression of soybean trypsin inhibitor gene in transgenic tobacco plants failed to confer resistance against damage caused byHelicoverpa armigera

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/024/04/0445-0452

    • Keywords

       

      Protease inhibitors; transgenic plant; heteroiogous gene expression; insect resistance

    • Abstract

       

      Helicoverpa armigera is a major pest of many tropical crop plants. Soybean trypsin inhibitor (SBTI) was highly effective against the proteolytic activity of gut extract of the insect. SBTI was also inhibitory to insect growth when present in artificial diet. The gene coding for SBTI was cloned from soybean (Glycine max, CVBirsa) and transferred to tobacco plants for constitutive expression. Young larvae ofH. armigera, fed on the leaves of the transgenic tobacco plants expressing high level of SBTI, however, maintained normal growth and development. The results suggest that in certain cases the trypsin inhibitor gene(s) may not be suitable candidates for developing insect resistant transgenic plants.

    • Author Affiliations

       

      Ashis Kumar Nandi1 2 3 Debabrata Basu1 Sampa Das1 2 Soumitra K Sen1

      1. IIT-BREF Biotek, Indian Institute of Technology, Kharagpur - 721 302, India
      2. Plant Molecular and Cellular Genetics, Bose Institute, P1/12, CIT Scheme VII-M, Calcutta - 700 054, India
      3. Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore - 560 012, India
    • Dates

       
  • Journal of Biosciences | News

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