• Ascribing functions to the lamin B receptor

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/024/03/0401-0408

    • Keywords

       

      Nuclear envelope; sterol biosynthesis; Neurospora ; membrane-heterochromatin interaction

    • Abstract

       

      This article reviews the research on the inner nuclear membrane protein lamin B receptor (LBR). It focuses on the biochemical and immunological evidence for an LBR; the cloning of chicken, rat and human LBR cDNAs and genomic sequences; the lamin B-, chromatin-, DNA- and NLS-binding properties of the N-terminal domain and its phosphorylation by different kinases; the sterol C-14 reductase activity of the C-terminal domain; the use of yeast two-hybrid screens and co-immunoprecipitation to identify interacting proteins; and the probing of nuclear assembly and disassembly in living cells with LBR-GFP fusion proteins. The article concludes by considering a scenario whereby LBR levels might even regulate gene expression.

    • Author Affiliations

       

      Durgadas P Kasbekar1

      1. Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India
    • Dates

       
  • Journal of Biosciences | News

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