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    • Keywords


      Human acrosin; seminal inhibitor; kinetics

    • Abstract


      A low molecular mass, naturally occurring acrosin inhibitor has been identified and purified (490-7-fold) from human semen, and kinetic studies have been performed on the association characteristics as well as for the determination of affinity constants (Ki values). The results show thatKi value (3.34 × 10−2) of the inhibitor towards human acrosin is almost three times lower than that of pancreatic trypsin, indicating a much higher specificity and inhibitory property for acrosin. The purified human seminal acrosin inhibitor has a molecular mass of 5.5 kDa and shows a single band using 10–20% gradient SDS PAGE. The work is of great significance for the development of more specific, nontoxic and irreversible inhibitors for human acrosin.

    • Author Affiliations


      Aditi Chatterjee1 Sagarika Kanjilal1 2 Asok K Bhattacharyya1

      1. Reproductive Biology Laboratory, Department of Biochemistry, Calcutta University College of Science, 35, Ballygunge Circular Road, Calcutta - 700 019, India
      2. Department of Veterinary Pathobiology, University of Minnesota, Twin Cities, MN - 55108, USA
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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