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      https://www.ias.ac.in/article/fulltext/jbsc/024/03/0295-0299

    • Keywords

       

      Comparative genome analysis; genotyping; biodiversity; endangered species; DNA probe; generic affinities

    • Abstract

       

      DNA typing using genome derived cloned probes may be conducted for ascertaining genetic affinities of closely related species. We analysed gaurBos gaurus, cattleBos indicus, buffaloBubalus bubalis, sheepOvis aries and goatCapra hircus DNA using buffalo derived cloned probe pDS5 carrying an array ofBamHI satellite fraction of 1378 base residues to uncover its genomic organization. Zoo-blot analysis showed that pDS5 does not cross hybridize with non-bovid animals and surprisingly with female gaur genomic DNA. The presence of pDS5 sequences in the gaur males suggests their possible location on the Y chromosome. Genotyping of pDS5 withBamHI enzyme detected mostly monomorphic bands in the bubaline samples and polymorphic ones in cattle and gaur giving rise to clad specific pattern. Similar typing withRsaI enzyme also revealed clad specific band pattern detecting more number of bands in buffalo and fewer in sheep, goat and gaur samples. Copy number variation was found to be prominent in cattle and gaur withRsaI typing. Our data based on matched band profiles (MBP) suggest that gaur is genetically closer to cattle than buffalo contradicting the age-old notion held by some that gaur is a wild buffalo. The pDS5 clone has a potential for estimating the generic and genetic relationship amongst closely related bovid species.

    • Author Affiliations

       

      Pritha Ray1 Supriya Gangadharan1 Munmun Chattopadhyay1 Anu Bashamboo1 Sunita Bhatnagar1 Pradeep Kumar Malik1 2 Sher Ali1

      1. Molecular Genetics Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi - 110 067, India
      2. Wildlife Institute of India, Chandrabani, Dehra Dun - 248 001, India
    • Dates

       
  • Journal of Biosciences | News

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