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Covalent enzyme electrodes; biosensors; mediated electron transfer

We have studied the glucose oxidase immobilized carbon paste electrodes in the presence and absence of small mediator molecules. We have used p-benzoquinone and riboflavin as mediators in our studies. The effect of mediator molecules on the electron transfer between the enzyme redox centre and the electrode surface was explained from the cyclic voltammograms and rotating disk electrode data. In the absence of oxygen, we have noted that the mediators play a central role in the electron transfer. We have also proposed a possible mechanism for the electron transfer from enzyme active site to the electrode surface via mediators, based on our observations.

D Savitri¹ Chanchal K Mitra¹

1. Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad - 500 046, India

Published

March 1999

Manuscript received

4 September 1998

Accepted

17 December 1998