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    • Keywords


      Streptomyces ; geosmin; MIB; curing agent; aerial mycelium; bald mutant; linear plasmid

    • Abstract


      Various species of actinomycetes and cyanobacteria can impart earthy/musty off-flavours to drinking water supplies and to pond-raised fish and other aquatic food animals. The genetic determinants for production of the most common off-flavour compounds [geosmin and 2-methylisoborneol (MIB)] have not been extensively studied. An attempt has been rrlade to study the genetics of production of these compounds was demonstrated by DNA-curing analysis. The effects of two curing agents [ethidium bromide (EB) and N-methyl-N′-nitro-N-nitrosoguanidine (NTG)] on tha loss of linear plasmid DNA and generation of bald mutants (no aerial mycelia) inStreptomyces halstedii andStereptomyces violaceusniger which produce geosmin and MIB, respectively, were observed. Production of earthy/musty odour was not eliminated, but was reduced by 55–95% in the plasmid cured strain. Data suggested that off-flavour production is likely chromosomally-encoded in theseStreptomyces isolates.

    • Author Affiliations


      Ismail Saadoun1 Ahmad Elbetieha1 Willard T Blevins1 2

      1. Department of Biological Sciences, Jordan University of Science and Technology, Irbid - 22110, Jordan
      2. Suncrest Laboratories, LLC, 9500 AL Hwy 14 W, Notasulga, AL - 36866, USA
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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