• Recombinant expression of human transforming growth factor-β isoforms in Chinese hamster ovary cells

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      https://www.ias.ac.in/article/fulltext/jbsc/023/05/0577-0583

    • Keywords

       

      Recombinant DNA; gene expression; growth factors; TGF-β; CHO cells

    • Abstract

       

      Transforming growth factor-βs (TGF-βs) are multi functional growth modulators implicated in several physiological processes which include embryogenesis, inflammation, immune-suppression, wound healing, carcinogenesis and cellular differentiation. For clinical use, recombinant expression of TGF-βs is the only practical source because of very low yields from natural sources. Here, we report the recombinant expression of human TGF-βl and TGF-β2 in a mammalian expression system using a high expression eukaryotic vector driven by a cytomegalovirus promoter. Expression levels are as high as 0.97 μg/ml of TGF-βl and 0.24 μg/ml of TGF-β2 in conditioned media, sufficient for purification without the need for amplification of the gene using methotrexate.

    • Author Affiliations

       

      B Ramani1 Paturu Kondaiah1

      1. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore - 560012, India
    • Dates

       
  • Journal of Biosciences | News

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