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    • Keywords


      Enhancing factor; phospholipase A2; inflammation; neutrophil; Paneth cells; EGF

    • Abstract


      Enhancing factor (EF), a mouse phospholipase A2 (PLA2), has been purified from the small intestines, based on its ability to increase the binding of epidermal growth factor in a radioreceptor assay. EF/PLA2 was found to be localized predominantly in the Paneth cells in the small intestines. Whether mouse intestinal EF/PLA2 is identical/similar to mouse secretory PLA2 was to be determined. Phospholipases are known to play a crucial role in the process of inflammation. This paper reports the presence of trace amounts of EF/PLA2 in the peritoneal exudate cells. Western blot analysis of the acid extracts showed the presence of a 14 kDa immunologically cross-reactive protein. RT-PCR analysis using EF specific primers amplified a ∼700 bp product which was further confirmed to be EF-specific by nested PCR analysis and sequencing. Presence of EF in the peritoneal exudate cells could be a unique mode of transport of growth factor modulator to the site of injury to aid in regeneration/cell proliferation of damaged tissue.

    • Author Affiliations


      Abhay R Redkar1 Shilpa Kadam1 Madhav G Deo1 Rita Mulherkar1

      1. Laboratory of Genetic Engineering, Cancer Research Institute Tata Memorial Centre Parel, Mumbai - 400012, India
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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