Yeast mitochondrial RNA polymerase was purified and resolved into 2 distinct fractions. Peak A was found to be nonspecific and exhibited characteristics of the core polymerase, whereas peak B exhibited characteristics of the holoenzyme.In vitro replication assays were carried out, using the peak B enzyme, the clonedori sequences and other DNA templates. It was found thatori 2 was the most efficient template for RNA polymerase primed DNA synthesis, as compared to all the other templates studied.
Volume 44 | Issue 5
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