• Immunoprophylactic studies on cell wall associated proteins ofMycobacterium tuberculosis H37Ra

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      https://www.ias.ac.in/article/fulltext/jbsc/022/01/0013-0021

    • Keywords

       

      Tuberculosis ; cell wall ; protein antigens ; vaccine

    • Abstract

       

      The cell wall protein peptidoglycan complex (CW-PPC) ofMycobacterium tuberculosis H37Ra was isolated through sequential extraction of lipids, carbohydrates and soluble proteins. CW-PPC emulsified in FIA was found to induce significant protection in mice against challenge with LD50 dose ofM. tuberculosis H37Rv. To identify the immunoprotective components of CW-PPC, the proteins in avid association with peptidogican were dissociated by chemical treatment with trifluoromethanesulthonic acid (CF3CO3H): anisole (2:1). Immunoreactivity of total (CW-Pr) as well as its component proteins i.e., 71, 60 and 45 kDa proteins of cell wall was studied in animals immunized with CW-Pr-FIA. The 71 kDa protein was found to be most immunoreactive giving higher T-cell sensitization and humoral responses. Further, immunization of mice with 71 kDa-FIA demonstrated enhanced T- and B- cell responses. Mice immunized with 71 kDa-FIA gave significantly higher protection (P ≤ 0.05) against intravenous challenge with LD50 dose ofM. tuberculosis H37Rv, than BCG immunized animals. The results indicate the potential of 71 kDa cell wall protein as a suitable candidate for Cthe subunit vaccine.

    • Author Affiliations

       

      Neelam Dhiman1 Indu Verma1 G K Khuller1

      1. Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh - 160 012, India
    • Dates

       
  • Journal of Biosciences | News

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