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    • Mapping of assembled epitopic regions of human chorionic gonadotropin reveals proximity of CTPα to the determinant loop β93–100

      N S Srilatha G S Murthy

      Volume 21 Issue 6 December 1996 pp 755-764

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/021/06/0755-0764

    • Keywords

       

      Epitope mapping; assembled epitopes; human chorionic gonadotropin; monoclonal antibodies; carboxy terminal region; single step solid phase radioimmunoassay

    • Abstract

       

      Three overlapping assembled epitopes of βhCG have been mapped using MAb probes and a single step solid phase radioimmunoassay. These epitopes have been shown to be at receptor binding region comprising of the loop region μ Cys93-Cys100. Importance of disulphide bonds in maintaining integrity of these epitopes is assessed. Two MAbs (INN 58 and INN 22) interact with the μ region as well as the β C-terminal peptide, while the other MAb INN 24 interacts with only the μ region. Cross-reactivity pattern with μhCG and hLH as well as the reported crystal structure of hCG substantiates the epitope identification. The results demonstrate utility of MAbs as probes in investigations on three-dimensional structure of gonadotropins

    • Author Affiliations

       

      N S Srilatha1 G S Murthy1

      1. Primate Research Laboratory, Centre for Reproductive Biology and Molecular Endocrinology, Indian Institute of Science, Bangalore - 560012, India

    • Dates

       
      Published
      December 1996
      Manuscript received
      5 June 1996
      Manuscript revised
      21 September 1996

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