• Interactions of 3′ terminal and 5′ terminal regions of physalis mottle virus genomic RNA with its replication complex

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    • Keywords


      Physalis mottle virus; replication; inhibition; SSRNA plant virus

    • Abstract


      Physalis mottle virus (PhMV) belongs to the tymogroup of positive-strand RNA viruses with a genome size of 6 kb. Crude membrane preparations from PhMV-infectedNicotiana glutinosa plants catalyzed the synthesis of PhMV genomic RNA from endogenously bound template. Addition of exogenous gnomic RNA enhanced the synthesis which was specifically inhibited by the addition of sense and antisense transcripts corresponding to 3′ terminal 242 nucleotides as well as the 5′ terminal 458 nucleotides of PhMV genomic RNA while yeast tRNA or ribosomal RNA failed to inhibit the synthesis. This specific inhibition suggested that the 5′ and 3′ non-coding regions of PhMV RNA might play an important role in viral replication

    • Author Affiliations


      V Srividhya1 H S Savithri1

      1. Department of Biochemistry, Indian Institute of Science, Bangalore - 560012, India
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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