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    • Keywords


      Prolactin; luteinizing hormone binding; Leydig cell

    • Abstract


      Effect of prolactin on the testicular luteinizing hormone binding was studied in a serum-free culture system. By the collagenase digestion of decapsulated testes taken out from 25-day-old rats, Leydig cells were isolated and cultured for 7 days in DME/F12 (1:1) medium supplemented with insulin, transferrin, epidermal growth factor, and gentamicin. The cultured cells exhibited the 3β-hydroxysteroid dehydrogenase activity. Hill plots constructed from the data of competition experiment showed that the dissociation constant (Kd) was 0.33 × 10–10M. The Kd value was approximately the same as the known value for the rat testicular homogenates. When the Leydig cells were cultured with ovine prolactin for the last 3 days of 7-day culture period, the binding of luteinizing hormone increased to 1.7-fold ofthat in the control group. From these results it is concluded that prolactin acts to up-regulate the binding of luteinizing hormone to rat testicular Leydig cells in serum-free culture

    • Author Affiliations


      Minoru Takase1 Kazuyoshi Tsutsui1 2 Seiichiro Kawashima1 3

      1. Laboratory for Amphibian Biology, Faculty of Science, Hiroshima University, Higashi-Hiroshima - 739, Japan
      2. Faculty of Integrated Arts and Science, Hiroshima University, Higashi-Hiroshima - 739, Japan
      3. Research Laboratory, Zenyaku Kogyo Co., Ltd., Tokyo - 178, Japan
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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