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    • Keywords


      Protein tyrosine phosphatase; nuclear phosphatase; liver regeneration; cell cycle; cell proliferation

    • Abstract


      The non-transmembrane protein tyrosine phosphatase, PTP-S, is located predominantly in the cell nucleus in association with chromatin. Here we have analysed the expression of PTP-S upon mitogenic stimulation and during cell division cycle. During liver regeneration after partial hepatectomy, PTP-S mRNA levels increased 16-fold after 6 h (G1 phase) and declined thereafter. Upon stimulation of serum starved cells in culture with serum, PTP-S mRNA levels increased reaching a maximum during late G1 phase and declined thereafter. No significant change in PTP-S RNA levels was observed in growing cells during cell cycle. PTP-S protein levels were also found to increase upon mitogenic stimulation. Upon serum starvation for 72 h, PTP-S protein disappears from the nucleus and is seen in the cytoplasm; after 96 h of serum starvation the PTP-S protein disappears from the nucleus as well as cytoplasm. Refeeding of starved cells for 6 h results in reappearance of this protein in the nucleus. Our results suggest a role of this phosphatase during cell proliferation.

    • Author Affiliations


      Sundaram Nambirajan1 R Sreek1 Antha Reddy1 Ghanshyam Swarup1

      1. Center for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India
    • Dates

  • Journal of Biosciences | News

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