• Expression of parasporal crystal protein (δ—endotoxin) gene(s) ofBacillus thuringiensis var.israelensis in sporogenic and asporogenic mutant strains ofBacillus cereus

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      https://www.ias.ac.in/article/fulltext/jbsc/019/02/0145-0153

    • Keywords

       

      Bacillus thuringiensis ; Bacillus cereus ; sporulation; δ-endotoxin; conjugation

    • Abstract

       

      The expression of parasporal crystal protein (δ-endotoxin) coding gene(s) ofBacillus thuringlensis var.israelensis and its association, if any, with sporulation was studied in sporogenicBacillus cereus and its asporogenic mutant strains. Five asporogenous mutants ofBacillus cereus blocked at different stages of sporulation, were isolated from a streptomycin-resistant strain, The transconjugants isolated from the plasmid transfer experiments betweenBacillus thuringiensis var.israelensis and streptomycin resistantBacillus cereus and its asporogenous mutants, showed larvicidal activity. The crystal protein gene(s) are, therefore, expressed both in sporulating and in non-sporulating mutant strains ofBacillus cereus suggesting that the expression of crystal protein gene(s), is independent of sporulation specific functions inBacillus cereus.

    • Author Affiliations

       

      Pramatha R Bhattacharya1

      1. Malaria Research Centre, 22 Sham NathMarg, Delhi - 110 054, India
    • Dates

       
  • Journal of Biosciences | News

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