• Characterization of mitochondrial DNA primase fromSaccharomyces cerevisiae

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    • Keywords

       

      Primase; yeast; mitochondria

    • Abstract

       

      DNA primase from yeast mitochondria was shown to have a molecular weight of 67 kDa by SDS-PAGE and an S value of 5.5. It was shown to have preference for SS mitochondrial DNA especially fragments containing origins of replication, as a template to initiate DNA replication. Further examination of the enzyme showed its possible association with a ribonucleotide moiety essential for enzyme activity.

    • Author Affiliations

       

      V Murthy1 K Pasupathy1

      1. Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Bombay - 400 085, India
    • Dates

       
  • Journal of Biosciences | News

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