DNA primase from yeast mitochondria was shown to have a molecular weight of 67 kDa by SDS-PAGE and an S value of 5.5. It was shown to have preference for SS mitochondrial DNA especially fragments containing origins of replication, as a template to initiate DNA replication. Further examination of the enzyme showed its possible association with a ribonucleotide moiety essential for enzyme activity.
Volume 44 | Issue 5
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