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      https://www.ias.ac.in/article/fulltext/jbsc/018/03/0319-0326

    • Keywords

       

      Urinary filarial antigen; immunodiagnosis; SDS-PAGE; immunoblot; ELISA

    • Abstract

       

      Urinary filarial antigen isolated from urine samples of microfilaraemic patients was analysed for its antigenic activity by immunoblotting and enzyme linked immunosorbent assay techniques. SDS-PAGE fractionation of urinary filarial antigen showed 11 protein bands, of which two showed reactivity with immunoglobulin-G fraction of filarial serum immunoglobulin in immunoblotting. Antigenic analysis of SDS-PAGE fractions of urinary filarial antigen by inhibition enzyme linked immunosorbent assay using filarial serum immunoglobulin-G andWuchereria bancrofti microfilarial excretory-secretory antigen revealed 3 fractions, numbers 5, 6 and 9 with significant activity. In indirect enzyme linked immunosorbent assay using fractions 5 and 6, filarial immunoglobulin-G antibody was detected in about 90% of microfilaraemics, 80% clinical filariasis and 20% of endemic normal individuals. Further, there was no phosphorylcholine epitope in these fractions. Fractions 5 and 6 can be a candidate antigens for the immunodiagnosis of filariasis.

    • Author Affiliations

       

      V Chenthamarakshan1 U M Padigel1 P Ramaprasad2 M V R Reddy1 B C Harinath1

      1. Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram - 442 102, India
      2. Lupin Laboratories Ltd., Mandideep - 462 042, India
    • Dates

       
  • Journal of Biosciences | News

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