• Bacillus thuringiensis crystal protein (δ-endotoxin) gene expression is independent of early sporulation specific functions

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    • Keywords

       

      Bacillus thuringiensis ; sporulation; crystal protein; δ-endotoxin; protease; DNAse

    • Abstract

       

      Bacillus thuringiensis produces a parasporal insecticidal crystal protein. The correlation between sporulation and crystal protein production inBacillus thuringiensis var.israelensis was studied. The strain was made resistant’against streptomycin (StR)-Acrystalliferous (Cry-) cured derivatives and asporogenous acrystalliferous (Spo Cry) mutants blocked at an early stage of sporulation were isolated. Plasmid transfer experiments were performed between StR Spo+ Cry+ (streptomycin sensitive sporogeneous crystalliferous) and StRR Spo+ Cry and also between Sts Spo+ Cry+ and StR Spo Cry strains. StR colonies were selected. Insect toxicity was exhibited by the StR isolates in both the cases. The process of crystal formation is, therefore, independent of early sporulative events.

    • Author Affiliations

       

      Pramatha R Bhattacharya1 2

      1. Department of Biotechnology, Jadavpur University, Calcutta - 700 032, India
      2. Malaria Research Centre, 22-Sham Nath Marg, Delhi - 110 054, India
    • Dates

       
  • Journal of Biosciences | News

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